概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZBTB40
  • 经测试应用
    适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 1140 and 1190 of human ZBTB40 (NP_055685.3)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab86330 was affinity purified using an epitope specific to ZBTB40 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 138 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 12 C2H2-type zinc fingers.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BC059177 antibody
    • C230087D24 antibody
    • Gm571 antibody
    • KIAA0478 antibody
    • MGC133098 antibody
    • MGC62412 antibody
    • mKIAA0478 antibody
    • RGD1309866 antibody
    • RP23 95O23.1 antibody
    • ZBT40_HUMAN antibody
    • ZBTB40 antibody
    • Zinc finger and BTB domain containing 40 antibody
    • Zinc finger and BTB domain containing protein 40 antibody
    • Zinc finger and BTB domain-containing protein 40 antibody
    • ZNF923 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZBTB40 with ab86330 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ZBTB40 antibody (ab86330) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysates at 50 µg
    Lane 2 : 293T whole cell lysates at 15 µg
    Lane 3 : 293T whole cell lysates at 5 µg

    Developed using the ECL technique

    Predicted band size : 138 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of ZBTB40 by Western Blot of Immunprecipitate.
    ab86330, at 1 µg/ml, staining ZBTB40 in HeLa whole cell lysate immunoprecipitated using ab86330 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with an exposure time of 3 minutes.

文献

ab86330 has not yet been referenced specifically in any publications.

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