Anti-ZBTB38抗体(ab50664)

概述

  • 产品名称Anti-ZBTB38抗体
    参阅全部 ZBTB38 一抗
  • 描述
    兔多克隆抗体to ZBTB38
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig
  • 免疫原

    A region within synthetic peptide: MSLSRDLKDD FHSDTVLSIL NEQRIRGILC DVTIIVEDTK FKAHSNVLAA, corresponding to N terminal amino acids 1-50 of Human ZBTB38

  • 阳性对照
    • HepG2 cell lysate and human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Peptide ELISA: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 135 kDa).

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

Anti-ZBTB38 antibody 图像

  • Anti-ZBTB38 antibody (ab50664) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1:100,000)

    Predicted band size : 135 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.Ab50664 (4-8ug/ml) staining human ZBTB38 in human kidney tissue by immunohistochemistry using paraffin embedded tissue. Epithelial cells of renal tubule (indicated with arrows) positively labelled.

Anti-ZBTB38 antibody (ab50664)参考文献

ab50664 has not yet been referenced specifically in any publications.

Product Wall

Thank you for forwrding your images. They will be very helpful for our investigation into the problem with this antibody.

Please keep me updated about the results after trying the replacement antibody, and let me know if there is anything els...

Read More

Thank you for contacting us and letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge replacement vial of ab50664 which should arrive tomorrow on the order ***. Please keep me updated about the re...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"