RabMAb

Anti-ZAP70 (phospho Y492)抗体[EP2291Y] (ab76306)

概述

  • 产品名称Anti-ZAP70 (phospho Y492)抗体[EP2291Y]
    参阅全部 ZAP70 一抗
  • 描述
    兔单克隆抗体[EP2291Y] to ZAP70 (phospho Y492)
  • 特异性ab76306 only detects ZAP70 phosphorylated on Tyrosine 492.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyr492 of human ZAP70

  • 阳性对照
    • Jurkat cell lysates, untreated or treated with pervanadate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IP 1/30.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
    • 组织特异性Expressed in T- and natural killer cells.
    • 疾病相关Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
    • 结构域The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
    • 翻译后修饰Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
    • 细胞定位Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC 2.7.10.2 antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Anti-ZAP70 (phospho Y492) antibody [EP2291Y] 图像

    • All lanes : Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306) at 1/20000 dilution

      Lane 1 : Jurkat cell lysates, untreated
      Lane 2 : Jurkat cell lysates, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    Anti-ZAP70 (phospho Y492) antibody [EP2291Y] (ab76306)参考文献

    This product has been referenced in:
    • Chiang YJ  et al. CBAP functions as a novel component in chemokine-induced ZAP70-mediated T-Cell adhesion and migration. PLoS One 8:e61761 (2013). WB . Read more (PubMed: 23620790) »

    See 1 Publication for this product

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