Anti-ZAP70 (phospho Y292)抗体[EPR1073] (ab76501)


  • 产品名称Anti-ZAP70 (phospho Y292)抗体[EPR1073]
    参阅全部 ZAP70 一抗
  • 描述
    兔单克隆抗体[EPR1073] to ZAP70 (phospho Y292)
  • 特异性ab76501 only detects ZAP70 when phosphorylated at Tyrosine 292.
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZAP70 (phospho Y292). The exact sequence is proprietary.

  • 阳性对照
    • WB: Jurkat cell lysate treated with pervanadate. ICC/IF: Untreated and pervanadate treated Jurkat cells. Flow Cyt: Untreated and pervanadate treated Jurkat cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-ZAP70 (phospho Y292) antibody (Alexa Fluor® 488) [EPR1073] (ab199244)
    Anti-ZAP70 (phospho Y292) antibody (Alexa Fluor® 647) [EPR1073] (ab199497)




Our Abpromise guarantee covers the use of ab76501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Predicted molecular weight: 70 kDa.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
    • 组织特异性Expressed in T- and natural killer cells.
    • 疾病相关Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
      Contains 1 protein kinase domain.
      Contains 2 SH2 domains.
    • 结构域The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
    • 翻译后修饰Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
    • 细胞定位Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 70 kDa zeta associated protein antibody
      • 70 kDa zeta-associated protein antibody
      • EC antibody
      • FLJ17670 antibody
      • FLJ17679 antibody
      • Selective T cell defect antibody
      • SRK antibody
      • STD antibody
      • Syk related tyrosine kinase antibody
      • Syk-related tyrosine kinase antibody
      • Truncated ZAP kinase antibody
      • Tyrosine protein kinase ZAP70 antibody
      • Tyrosine-protein kinase ZAP-70 antibody
      • TZK antibody
      • ZAP 70 antibody
      • ZAP70 antibody
      • ZAP70_HUMAN antibody
      • Zeta chain associated protein kinase 70kD antibody
      • Zeta chain associated protein kinase 70kDa antibody
      • Zeta chain associated protein kinase 70kDa isoform 1 antibody
      • Zeta chain associated protein kinase 70kDa isoform 2 antibody
      • Zeta chain TCR associated protein kinase 70kD antibody
      • Zeta chain TCR associated protein kinase 70kDa antibody
      see all

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] 图像

    • All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/5000 dilution

      Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysates
      Lane 2 : Jurkat (human acute T cell leukemia) treated with pervanadate whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of varying amounts of ZAP70 (pY292) phospho peptide (Lane 1) and ZAP70 non-phospho peptide (Lane 2) using ab76501 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Exposure time: 3 minutes.

    • Immunocytochemistry/Immunofluorescence analysis of untreated and pervanadate treated Jurkat (human acute T cell leukemia) cells labelling ZAP70 (phospho Y292) with ab76501 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Treated samples were starved overnight followed by incubation with 1mM pervanadate for 30 minutes. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Negative control using secondary antibody only.

      Image shows increased cytoplasmic staining after pervanadate treatment.

    • Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).

    Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)参考文献

    ab76501 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Jurkat cell line)
    Loading amount 40 µg
    Specification Jurkat cell line
    Treatment 5mM H2O2 for 5min
    Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

    Abcam user community

    Verified customer

    提交于 Dec 07 2012

    Thank you very much for submitting your valuable reveiw of ab76501.
    I'm writing to follow-up with you, as the results were below average and we do guarantee the results of this antibody in Western blot with human samples. If the results are not sa...

    Read More