概述
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产品名称Anti-ZAP70 (phospho Y292)抗体[EPR1073]
参阅全部 ZAP70 一抗 -
描述兔单克隆抗体[EPR1073] to ZAP70 (phospho Y292)
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宿主Rabbit
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特异性ab76501 only detects ZAP70 when phosphorylated at Tyrosine 292.
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经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性与反应: Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ZAP70 (phospho Y292). The exact sequence is proprietary.
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阳性对照
- WB: Jurkat cell lysate treated with pervanadate. ICC/IF: Untreated and pervanadate treated Jurkat cells. Flow Cyt: Untreated and pervanadate treated Jurkat cells.
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常规说明
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR1073
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab76501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000 - 1/10000. Predicted molecular weight: 70 kDa. | |
| Flow Cyt | 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | 1/250 - 1/500. |
靶标
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功能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
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组织特异性Expressed in T- and natural killer cells.
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疾病相关Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
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序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Contains 1 protein kinase domain.
Contains 2 SH2 domains. -
结构域The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
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翻译后修饰Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
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细胞定位Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
- Information by UniProt
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数据库链接
- Entrez Gene: 7535 Human
- Omim: 176947 Human
- SwissProt: P43403 Human
- Unigene: 234569 Human
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别名
- 70 kDa zeta associated protein antibody
- 70 kDa zeta-associated protein antibody
- EC 2.7.10.2 antibody
see all
图片
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![Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](http://www.abcam.cn/ps/products/76/ab76501/Images/ab76501-252760-ab76501WB.jpg)
Western blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501) at 1/5000 dilution
Lane 1 : Untreated Jurkat (human acute T cell leukemia) whole cell lysates
Lane 2 : Jurkat (human acute T cell leukemia) treated with pervanadate whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](http://www.abcam.cn/ps/products/76/ab76501/Images/ab76501-252761-ab76501Dot.jpg)
Dot Blot - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)Dot blot analysis of varying amounts of ZAP70 (pY292) phospho peptide (Lane 1) and ZAP70 non-phospho peptide (Lane 2) using ab76501 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](http://www.abcam.cn/ps/products/76/ab76501/Images/ab76501-252762-ab76501ICCIF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)Immunocytochemistry/Immunofluorescence analysis of untreated and pervanadate treated Jurkat (human acute T cell leukemia) cells labelling ZAP70 (phospho Y292) with ab76501 at a dilution of 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Treated samples were starved overnight followed by incubation with 1mM pervanadate for 30 minutes. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Negative control using secondary antibody only.
Image shows increased cytoplasmic staining after pervanadate treatment.
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![Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)](http://www.abcam.cn/ps/datasheet/Images/76/ab76501/ab76501 FC.JPG)
Flow Cytometry - Anti-ZAP70 (phospho Y292) antibody [EPR1073] (ab76501)Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).
实验方案
文献
ab76501 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"