Anti-ZAP70 (phospho Y292)抗体(ab12868)

概述

  • 产品名称Anti-ZAP70 (phospho Y292)抗体
    参阅全部 ZAP70 一抗
  • 描述
    兔多克隆抗体to ZAP70 (phospho Y292)
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from a region of human ZAP70 that contains tyrosine 292 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • 阳性对照
    • Jurkat cells treated with hydrogen peroxide.
  • 常规说明Centrifuge before opening to settle contents.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Dulbecco's PBS without magnesium and calcium, 50% Glycerol, PBS, 1mg/ml BSA, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated ZAP70. The final product is generated by affinity chromatography using a ZAP70-derived peptide that is phosphorylated at threonine 292.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 74.3 kDa.

靶标

  • 功能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • 组织特异性Expressed in T- and natural killer cells.
  • 疾病相关Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • 结构域The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • 翻译后修饰Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • 细胞定位Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC 2.7.10.2 antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Anti-ZAP70 (phospho Y292) antibody 图像



  • Predicted band size : 74.3 kDa


    Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells left untreated (1) or treated with hydrogen peroxide (2-6) were either left untreated (1-5) or treated with lambda phosphatase (6). Lysates were then incubated with ab12868 following prior incubation with: no peptide (1, 6), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine-containing peptide (4) or, the phosphopeptide immunogen (5). The data show that only the peptide corresponding to ZAP70 [phospho Y292] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-ZAP70 (phospho Y292) antibody (ab12868)参考文献

This product has been referenced in:
  • Haas A  et al. Intracellular Phospho-Flow cytometry reveals novel insights into TCR proximal signaling events. A comparison with Western blot. Cytometry A 73:799-807 (2008). WB ; Human . Read more (PubMed: 18548611) »

See 1 Publication for this product

Product Wall

Thank you again for your patience, I have just heard back from the originator of this antibody. Are you not seeing any signal at all? The originator said that it worked well for them on an endogenous system. Also, ZAP-70 tyrosine 292 is the negativ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab12868. You mentioned that you are using Jurkat cell lysates; did you treat the lysates with hydrogen peroxide? Jurkat cells treated with hydrogen peroxide are ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"