RabMAb

Anti-ZAP70抗体[YE291] (ab32429)

概述

  • 产品名称Anti-ZAP70抗体[YE291]
    参阅全部 ZAP70 一抗
  • 描述
    兔单克隆抗体[YE291] to ZAP70
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ZAP70 aa 550-650 (C terminal).

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human lymph node tissue.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab32429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

靶标

  • 功能Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
  • 组织特异性Expressed in T- and natural killer cells.
  • 疾病相关Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • 结构域The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.
  • 翻译后修饰Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
  • 细胞定位Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70 kDa zeta associated protein antibody
    • 70 kDa zeta-associated protein antibody
    • EC 2.7.10.2 antibody
    • FLJ17670 antibody
    • FLJ17679 antibody
    • Selective T cell defect antibody
    • SRK antibody
    • STD antibody
    • Syk related tyrosine kinase antibody
    • Syk-related tyrosine kinase antibody
    • Truncated ZAP kinase antibody
    • Tyrosine protein kinase ZAP70 antibody
    • Tyrosine-protein kinase ZAP-70 antibody
    • TZK antibody
    • ZAP 70 antibody
    • ZAP70 antibody
    • ZAP70_HUMAN antibody
    • Zeta chain associated protein kinase 70kD antibody
    • Zeta chain associated protein kinase 70kDa antibody
    • Zeta chain associated protein kinase 70kDa isoform 1 antibody
    • Zeta chain associated protein kinase 70kDa isoform 2 antibody
    • Zeta chain TCR associated protein kinase 70kD antibody
    • Zeta chain TCR associated protein kinase 70kDa antibody
    see all

Anti-ZAP70 antibody [YE291] 图像

  • Anti-ZAP70 antibody [YE291] (ab32429) at 1/500 dilution + Jurkat cell lysate

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • Ab32429, at a 1/100 dilution, staining ZAP70 in paraffin embedded human lymph node tissue by immunohistochemistry.
  • ab32429 showing positive staining in Normal tonsil tissue.

  • ab32429 showing negative staining in Normal liver tissue.

  • ab32429 showing negative staining in Normal heart tissue.

  • ab32429 showing negative staining in Normal kidney tissue.

Anti-ZAP70 antibody [YE291] (ab32429)参考文献

ab32429 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"