Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 235-265 of Human YTHDF3.
A549 cell lysate; Human skeletal muscle tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab103328 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 64 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
Contains 1 YTH domain.
Information by UniProt
YTH domain family 3 antibody
YTH domain family member 3 antibody
Western blot - YTHDF3 antibody (ab103328)
Anti-YTHDF3 antibody (ab103328) at 1/100 dilution + A549 cell lysate at 35 µg
Predicted band size : 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - YTHDF3 antibody (ab103328)
ab103328, at 1/50 dilution, staining YTHDF3 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.
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