antigen sequence, corresponding to internal sequence amino acids 69-160 (LVSKPLSSSV SNNKRIVSTK GKSATEYKNE EYQRSERNKR LDADRKIRLS SSASREPYKN QPEKTCVRKR DPERRAKSPT PDGSERIGLE VD) of Human YTHDC1
Human esophagus tissue; RT-4 and U-251 MG cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
May be part of a signal transduction pathway that influences splice site selection.
Contains 1 YTH domain.
Nucleus. Localizes to a novel subnuclear structure, the YT bodies.
Information by UniProt
Putative splicing factor YT521 antibody
splicing factor YT521-B antibody
Immunocytochemistry/ Immunofluorescence - Anti-YTHDC1 antibody (ab122340)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and plasma membrane. Recommended concentration of ab122340 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YTHDC1 antibody (ab122340)
ab122340, at a 1/400 dilution, staining YTHDC1 in paraffin-embedded Human esophagus tissue by immunohistochemistry.
Western blot - Anti-YTHDC1 antibody (ab122340)
All lanes : Anti-YTHDC1 antibody (ab122340) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human plasma Lane 4 : Human liver lysate Lane 5 : Human tonsil lysate Developed using the ECL technique
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-YTHDC1 antibody(ab122340)
Immunohistochemical staining of Human fallopian tube shows strong nuclear positivity in glandular cells using ab122340
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