概述

  • 产品名称
  • 描述
    兔多克隆抗体to YL1
  • 特异性
    Detects endogenous levels of total YL1 protein.
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal region of human YL1

  • 阳性对照
    • Human breast carcinoma tissue RAW264.7 cell extract NIH/3T3 cell extract

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
  • 序列相似性
    Belongs to the VPS72/YL1 family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CFL1 antibody
    • HGNC11644 antibody
    • Protein YL-1 antibody
    • Swc2 antibody
    • TCFL1 antibody
    • Transcription factor like 1 (YL1) antibody
    • Transcription Factor Like 1 antibody
    • Transcription factor-like 1 antibody
    • Vacuolar protein sorting 72 (yeast) antibody
    • Vacuolar protein sorting 72 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 72 homolog antibody
    • Vacuolar protein sorting-associated protein 72 homolog antibody
    • VPS72 antibody
    • VPS72_HUMAN antibody
    • YL-1 antibody
    • YL1 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab72506 at 1/50 dilution. Right panel was also treated with immunising peptide.
  • All lanes : Anti-YL1 antibody (ab72506) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : NIH 3T3 cell extract
    Lane 3 : NIH 3T3 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 41 kDa
    Additional bands at : 30 kDa,48 kDa,50 kDa,76 kDa,78 kDa,87 kDa. We are unsure as to the identity of these extra bands.

文献

ab72506 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Kidney cell line)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Kidney cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Dec 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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