Detects endogenous levels of total YL1 protein.
Synthetic peptide derived from an internal region of human YL1
- Human breast carcinoma tissue
RAW264.7 cell extract
NIH/3T3 cell extract
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity-chromatography using
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
||Use at an assay dependent concentration.
Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.
Belongs to the VPS72/YL1 family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- CFL1 antibody
- HGNC11644 antibody
- Protein YL-1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YL1 antibody (ab72506)
Immunohistochemical analysis of paraffin-embedded
human breast carcinoma tissue using ab72506 at 1/50 dilution. Right panel was also treated with immunising peptide.
Western blot - Anti-YL1 antibody (ab72506)
All lanes : Anti-YL1 antibody (ab72506) at 1/500 dilution
Lane 1 : RAW264.7 cell extract
Lane 2 : NIH 3T3 cell extract
Lane 3 : NIH 3T3 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 30 kDa, 48 kDa, 50 kDa, 76 kDa, 78 kDa, 87 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
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