概述

  • 产品名称
  • 描述
    兔多克隆抗体to YL1
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Baboon, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 50-100 of Human YL1 (NP_005988.1)

  • 阳性对照
    • Whole cell lysate from HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab112055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

Anti-YL1 antibody 图像

  • Immunoprecipitation of YL1. Samples: 1mg of whole cell lysate from HeLa cells(20% of IP loaded). Antibodies: Affinity purified rabbit anti-YL1 antibody (ab112055) was used at 1 µg/ml. Lane 1- YL1 was also immunoprecipitated by rabbit anti-YL1 antibody (ab112055) at 6 µg/mg lysate. Lane 2- YL1 was immunoprecipitated by an rabbit anti-YL1 antibody that recognizes a downstream epitope. 3- Rabbit IgG control.Detection: Chemiluminescence with exposure time of 10 seconds.
  • All lanes : Anti-YL1 antibody (ab112055) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 41 kDa

Anti-YL1 antibody (ab112055)参考文献

ab112055 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Melanoma)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
3000 cells
Specification
Melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Nov 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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