RabMAb

Anti-Yes1抗体[EPR3173] (ab109265)

概述

  • 产品名称Anti-Yes1抗体[EPR3173]
    参阅全部 Yes1 一抗
  • 描述
    兔单克隆抗体[EPR3173] to Yes1
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Yes1.

  • 阳性对照
    • WB: U-87MG, SW480 and Jurkat cell lysates IHC-P: Human colon tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Yes1 antibody (Alexa Fluor® 488) [EPR3173] (ab208381)

    Anti-Yes1 antibody (Alexa Fluor® 647) [EPR3173] (ab208382)

性能

应用

Our Abpromise guarantee covers the use of ab109265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Autophosphorylated.
    • 细胞定位Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies.
    • Information by UniProt
    • 数据库链接
    • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 别名
      • c Yes antibody
      • Cellular Yes 1 protein antibody
      • Cellular Yes1 protein antibody
      • HsT441 antibody
      • P61 YES antibody
      • p61-Yes antibody
      • Proto oncogene tyrosine protein kinase YES antibody
      • Proto-oncogene c-Yes antibody
      • Tyrosine-protein kinase Yes antibody
      • v yes 1 Yamaguchi sarcoma viral oncogene homolog 1 antibody
      • Viral oncogene yes 1 homolog 1 antibody
      • Viral oncogene yes1 homolog 1 antibody
      • Yamaguchi sarcoma oncogene antibody
      • Yes 1 antibody
      • Yes antibody
      • YES proto oncogene 1, Src family tyrosine kinase antibody
      • YES_HUMAN antibody
      • YES1 antibody
      • YES1 proto oncogene, Src family tyrosine kinase antibody
      see all

    Anti-Yes1 antibody [EPR3173] 图像

    • ab109265 at 1/50 dilution staining Yes1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
    • All lanes : Anti-Yes1 antibody [EPR3173] (ab109265) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 61 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    Anti-Yes1 antibody [EPR3173] (ab109265)参考文献

    ab109265 has not yet been referenced specifically in any publications.

    Product Wall


    I have included the antigen retrieval protocol below:

    Antigen Retrieval Solution: 0.01M Sodium Citrate Buffer, pH 6.0

    Antigen Retrieval

    Immerse slides into staining dish containing Antigen Retrieval Solution.

    Pl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"