Anti-YB1 (phospho S102)抗体(ab74162)


  • 产品名称Anti-YB1 (phospho S102)抗体
    参阅全部 YB1 一抗
  • 描述
    兔多克隆抗体to YB1 (phospho S102)
  • 经测试应用适用于: IHC-P, WB, Agglutinationmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human YB1, phosphorylated at S102.

    (Peptide available as ab74644.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; A549; MCF7; HEK293 Whole Cell Lysate Transiently Overexpressing YB1.




Our Abpromise guarantee covers the use of ab74162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 36 kDa).Can be blocked with YB1 (phospho S102) peptide (ab215269).
Agglutination Use at an assay dependent concentration.


  • 功能Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.
    The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
  • 序列相似性Contains 1 CSD (cold-shock) domain.
  • 翻译后修饰Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.
    In the absence of phosphorylation the protein is retained in the cytoplasm.
    Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
  • 细胞定位Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BP 8 antibody
    • CBF-A antibody
    • CCAAT binding transcription factor I subunit A antibody
    • CCAAT-binding transcription factor I subunit A antibody
    • CSDA2 antibody
    • CSDB antibody
    • DBPB antibody
    • DNA binding protein B antibody
    • DNA-binding protein B antibody
    • EFI-A antibody
    • Enhancer factor I subunit A antibody
    • MDR NF1 antibody
    • MGC104858 antibody
    • MGC110976 antibody
    • MGC117250 antibody
    • NSEP 1 antibody
    • NSEP1 antibody
    • Nuclease sensitive element binding protein 1 antibody
    • Nuclease-sensitive element-binding protein 1 antibody
    • p50 antibody
    • Q15905 antibody
    • Y-box binding protein 1 antibody
    • Y-box transcription factor antibody
    • Y-box-binding protein 1 antibody
    • YB 1 antibody
    • YB-1 antibody
    • YBOX1_HUMAN antibody
    • YBX 1 antibody
    • ybx1 antibody
    see all

Anti-YB1 (phospho S102) antibody 图像

  • All lanes : Anti-YB1 (phospho S102) antibody (ab74162) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Irradiated Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human YB1 (phospho S102) peptide (ab74644) at 1 µg/ml
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Irradiated Whole Cell Lysate with Human YB1 (phospho S102) peptide (ab74644) at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human YB1 peptide (ab74645) at 1 µg/ml
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Irradiated Whole Cell Lysate with Human YB1 peptide (ab74645) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Additional bands at : 50/55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • IHC image of YB1 (phospho S102) staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab74162, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-YB1 (phospho S102) antibody (ab74162)参考文献

ab74162 has not yet been referenced specifically in any publications.

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The only alternative antibody to YB1 (phospho S102) is ab138654 which has been tested in WB and ELISA and with human samples. It is predicted to react with mouse and rat, but not guaranteed.

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