概述

  • 产品名称Anti-YAP1抗体
    参阅全部 YAP1 一抗
  • 描述
    小鼠多克隆抗体to YAP1
  • 特异性This antibody reacts with Yes associated protein 1.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant fusion protein:

    LDPRLDPRFAMNQRISQSAP

    , corresponding to amino acids 259-278 of Human Yes Associated Protein 1

  • 阳性对照
  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab52708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 32.2 kDa (predicted molecular weight: 49 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  • 组织特异性Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).
  • 序列相似性Belongs to the YORKIE family.
    Contains 2 WW domains.
  • 翻译后修饰Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage.
  • 细胞定位Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 65 kDa Yes associated protein antibody
    • 65 kDa Yes-associated protein antibody
    • COB1 antibody
    • YAp 1 antibody
    • YAP 65 antibody
    • YAP antibody
    • YAP1 antibody
    • YAP1_HUMAN antibody
    • YAP2 antibody
    • YAP65 antibody
    • yes -associated protein delta antibody
    • Yes associated protein 1 65kDa antibody
    • Yes associated protein 1 antibody
    • Yes associated protein 2 antibody
    • yes associated protein beta antibody
    • YKI antibody
    • Yorkie homolog antibody
    see all

Anti-YAP1 antibody 图像

  • All lanes : Anti-YAP1 antibody (ab52708) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein tagged).

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 49 kDa
    Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

Anti-YAP1 antibody (ab52708)参考文献

ab52708 has not yet been referenced specifically in any publications.

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