概述

  • 产品名称Anti-Y14抗体
    参阅全部 Y14 一抗
  • 描述
    兔多克隆抗体to Y14
  • 特异性This antibody reacts with Y14
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM, corresponding to N terminal amino acids 1-50 of Human Y14

  • 阳性对照
    • WB: Jurkat cell lysate IHC-P: Human heart tissue

性能

应用

Our Abpromise guarantee covers the use of ab50570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The heterodimer MAGOH-RBM8A interacts with PYM that function to enhance the translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Remains associated with mRNAs in the cytoplasm until the mRNAs engage the translation machinery. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Complex with MAGOH is a component of the nonsense mediated decay (NMD) pathway.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the RBM8A family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 细胞定位Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Binder of OVCA1 1 antibody
    • Binder of OVCA1-1 antibody
    • BOV 1 antibody
    • BOV 1A antibody
    • BOV 1B antibody
    • BOV 1C antibody
    • BOV-1 antibody
    • BOV1 antibody
    • BOV1A antibody
    • BOV1B antibody
    • BOV1C antibody
    • HSPC 114 antibody
    • HSPC114 antibody
    • MDS 014 antibody
    • MDS014 antibody
    • RBM 8 antibody
    • RBM 8A antibody
    • RBM 8B antibody
    • RBM8 antibody
    • rbm8a antibody
    • RBM8A_HUMAN antibody
    • RBM8B antibody
    • Ribonucleoprotein RBM 8 antibody
    • Ribonucleoprotein RBM 8A antibody
    • Ribonucleoprotein RBM8 antibody
    • Ribonucleoprotein RBM8A antibody
    • RNA binding motif protein 8 antibody
    • RNA binding motif protein 8A antibody
    • RNA binding motif protein 8B antibody
    • RNA binding protein 8A antibody
    • RNA binding protein Y14 antibody
    • RNA-binding motif protein 8A antibody
    • RNA-binding protein 8A antibody
    • RNA-binding protein Y14 antibody
    • ZNRP antibody
    • ZRNP 1 antibody
    • ZRNP1 antibody
    see all

Anti-Y14 antibody 图像

  • Lane 1 :
    Lane 2 : Anti-Y14 antibody (ab50570) at 1.25 µg/ml

    Lane 1 : MARKER
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • ab50570 at 4µg/ml staining Y14 in myocardial cells of human heart.

Anti-Y14 antibody (ab50570)参考文献

ab50570 has not yet been referenced specifically in any publications.

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