Y-27632 dihydrochloride,Rho kinase抑制剂(ab120129)
Key features and details
- Selective Rho kinase inhibitor
- CAS Number: 129830-38-2
- Soluble in water to 100 mM and in DMSO to 100mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Y-27632 dihydrochloride,Rho kinase抑制剂 -
描述
Selective Rho kinase抑制剂 -
生物学描述
Y-27632 is a research tool with a wide variety of biological effects, mainly used in the field of stem cell research for maintenance and differentiation. Commonly used as 3D growth matrix component; also a component of brain organoid differentiation media.
How others have used Y-27632 in their research:
Maintenance of tissue culture of iPSCs; 10 μM Y-27632 for 24 hr; Montefiori et al (2018)
Reprogramming of breast cancer cells to induced progenitor-like cells (iPLs) ; 2 μM Y27632 ; Yuan et al (2018)
Differentiation - Y-27632 enables human embryonic stem cells to survive and differentiate into cortical and basal telencephalic progenitors; Watanabe et al (2007)
*Also available in simple stock solutions (ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
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CAS编号
129830-38-2 -
化学结构
性能
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化学名称
(R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride -
分子量
320.26 -
分子式
C14H21N3O.2HCl -
PubChem识别号
9901617 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 2 years. -
溶解度概述
Soluble in water to 100 mM and in DMSO to 100mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cl.Cl.O=C(Nc1ccncc1)[C@@H]2CC[C@H](CC2)[C@@H](C)N -
来源
Synthetic
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研究领域
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120129于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120129, Y-27632 dihydrochloride, Rho kinase inhibitor
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A: Serum-starved human TM cells were incubated on chamber glass slides coated with recombinant human Gal8 in the presence of the ROCK inhibitor ab120129, at different concentrations. After 2 hr, cells were stained with rhodamine-labeled phalloidin, and cells with robust stress fibers were enumerated. Data are expressed as mean±SEM.
B: Cells were treated with an 120129 (Y27632) at 20 µM, stained with rhodamine-labeled phalloidin, and random fields were photographed. Note that cells treated with ab120129 are not spread and exhibit dendrite-like structures.
Credit: Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400
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ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (120)
ab120129 被引用在 120 文献中.
- Mensah MA et al. Aberrant phase separation and nucleolar dysfunction in rare genetic diseases. Nature 614:564-571 (2023). PubMed: 36755093
- Schaafsma P et al. Role of immediate early genes in the development of salivary gland organoids in polyisocyanopeptide hydrogels. Front Mol Biosci 10:1100541 (2023). PubMed: 36818041
- Daskoulidou N et al. Complement receptor 1 is expressed on brain cells and in the human brain. Glia 71:1522-1535 (2023). PubMed: 36825534
- Shutova MS et al. Inflammation modulates intercellular adhesion and mechanotransduction in human epidermis via ROCK2. iScience 26:106195 (2023). PubMed: 36890793
- Yang H et al. Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery. JCI Insight 8:N/A (2023). PubMed: 37014712