Anti-XYLT2抗体(ab81311)

概述

  • 产品名称Anti-XYLT2抗体
    参阅全部 XYLT2 一抗
  • 描述
    兔多克隆抗体to XYLT2
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region of human XYLT2 (NP_071450).

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Probably catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes (By similarity). Its enzyme activity has not been demonstrated.
  • 组织特异性Widely expressed. Expressed at higher level in kidney and pancreas.
  • 通路Glycan metabolism; chondroitin sulfate biosynthesis.
    Glycan metabolism; heparan sulfate biosynthesis.
  • 序列相似性Belongs to the glycosyltransferase 14 family. XylT subfamily.
  • 翻译后修饰Contains disulfide bonds.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Peptide O xylosyltransferase 1 antibody
    • Peptide O-xylosyltransferase 1 antibody
    • protein xylosyltransferase 2 antibody
    • PXYLT2 antibody
    • UDP-D-XYLOSE:PROTEOGLYCAN CORE PROTEIN BETA-D-XYLOSYLTRANSFERASE 2 antibody
    • UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase antibody
    • XT II antibody
    • XT-II antibody
    • XT2 antibody
    • Xylosyltransferase 2 antibody
    • Xylosyltransferase II antibody
    • xylT II antibody
    • XylT-II antibody
    • XYLT2 antibody
    • XYLT2_HUMAN antibody
    see all

Anti-XYLT2 antibody 图像

  • Anti-XYLT2 antibody (ab81311) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Muscle Lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 97 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-XYLT2 antibody (ab81311)参考文献

ab81311 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Kidney, epithelial)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 30 µg
Treatment Non treated, High glucose (30mM), high glucose + metformin (1mM) for 24 hrs
Specification Kidney, epithelial
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. srikanth

Verified customer

提交于 Mar 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"