The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 108 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Catalyzes the first step in biosynthesis of glycosaminoglycan. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes.
组织特异性Widely expressed. Expressed at higher level in placenta, kidney and pancreas. Weakly expressed in skeletal muscle.
序列相似性Belongs to the glycosyltransferase 14 family. XylT subfamily.
翻译后修饰Contains 7 disulfide bonds. N-glycosylated.
细胞定位Endoplasmic reticulum membrane. Golgi apparatus membrane. Secreted. Some fraction is also found in the extracellular space together with chondroitin sulfate proteoglycans, suggesting that it can be secreted.