概述

  • 产品名称Anti-XRN2抗体
    参阅全部 XRN2 一抗
  • 描述
    兔多克隆抗体to XRN2
  • 经测试应用适用于: WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 25 and 75 of human XRN2 (NP_036387.2).

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab72284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/2000 - 1/10000. Detects bands of approximately 109 & 115 kDa (predicted molecular weight: 109 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
    • 细胞定位Nucleus, nucleolus.
    • 数据库链接
    • 别名
      • 5' 3' exoribonuclease 2 antibody
      • DHM1 like protein antibody
      • DHP protein antibody
      • XRN 2 antibody

    Anti-XRN2 antibody 图像

    • All lanes : Anti-XRN2 antibody (ab72284) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 109 kDa
      Observed band size : 109,115 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Anti-XRN2 antibody (ab72284)参考文献

    This product has been referenced in:
    • Ternette N  et al. Label-free quantitative proteomics reveals regulation of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and 5'-3'-exoribonuclease 2 (XRN2) during respiratory syncytial virus infection. Virol J 8:442 (2011). WB ; Human . Read more (PubMed: 21933386) »

    See 1 Publication for this product

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