Anti-XRCC4抗体(ab58468)

概述

  • 产品名称Anti-XRCC4抗体
    参阅全部 XRCC4 一抗
  • 描述
    兔多克隆抗体to XRCC4
  • 特异性ab58468 detects endogenous levels of total XRCC4 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from internal sequence of human XRCC4

  • 阳性对照
    • WB: Extracts from COLO205 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab58468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
ELISA 1/20000.

靶标

  • 功能Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the XRCC4 family.
  • 翻译后修饰Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody 图像

  • All lanes : Anti-XRCC4 antibody (ab58468) at 1/500 dilution

    Lane 1 : extracts from COLO205 cells with no immunising peptide
    Lane 2 : extracts from COLO205 cells with immunising peptide


    Predicted band size : 38 kDa

Anti-XRCC4 antibody (ab58468)参考文献

ab58468 has not yet been referenced specifically in any publications.

Product Wall



We have only tested this antibody in ELISA against the immunogenic peptide, so unfortunately we do not have any data from testing with cell lysates indicating the titer of XRCC4.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"