Anti-XRCC4抗体[4H9] (ab118008)

概述

  • 产品名称Anti-XRCC4抗体[4H9]
    参阅全部 XRCC4 一抗
  • 描述
    小鼠单克隆抗体[4H9] to XRCC4
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Protein expressed in 293T cells transfected with Human XRCC4 expression vector.

  • 阳性对照
    • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4; Human breast adenocarcinoma, colon, colon adenocarcinoma, kidney, lung, ovary, ovary adenocarcinoma, thyroid, thyroid carcinoma, endometrium, endometrium adenocarcinoma, prostate carcinoma or bladder carcinoma tissues.
  • 常规说明Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaw cycles.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 50% Glycerol, 48% PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab118008 is purified from Mouse ascite fluid by affinity chromatography.
  • 克隆单克隆
  • 克隆编号4H9
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 38 kDa.
IHC-P 1/50.

靶标

  • 功能Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the XRCC4 family.
  • 翻译后修饰Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
    Monoubiquitinated.
    Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
    • DNA repair protein XRCC4 antibody
    • SSMED antibody
    • X ray repair complementing defective repair in Chinese hamster cells 4 antibody
    • X ray repair cross complementing 4 antibody
    • X ray repair cross complementing protein 4 antibody
    • X-ray repair cross-complementing protein 4 antibody
    • XRCC 4 antibody
    • XRCC4 antibody
    • XRCC4_HUMAN antibody
    see all

Anti-XRCC4 antibody [4H9] 图像

  • All lanes : Anti-XRCC4 antibody [4H9] (ab118008) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY XRCC4 cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 38 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218029)
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Colon tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Colon adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Kidney tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Lung tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Ovary tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Thyroid tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Thyroid carcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human endometrium tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Prostate carcinoma tissue by Immunohistochemistry.
  • ab118008 at 1/50 dilution, staining XRCC4 in Paraffin- embedded Human Bladder carcinoma tissue by Immunohistochemistry.

Anti-XRCC4 antibody [4H9] (ab118008)参考文献

ab118008 has not yet been referenced specifically in any publications.

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