Anti-XRCC3抗体[XRCC3 10F1/6] (ab20254)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-XRCC3抗体[XRCC3 10F1/6]
参阅全部 XRCC3 一抗 -
描述小鼠单克隆抗体[XRCC3 10F1/6] to XRCC3
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宿主Mouse
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
Full length protein (human) which has been his-tagged, overexpressed in E.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page.
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阳性对照
- Does not recognise XRCC3 in HeLa total extracts- suggest nuclear extracts.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: None
Constituents: PBS -
Concentration information loading... -
克隆单克隆
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克隆编号XRCC3 10F1/6
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骨髓瘤Sp2/0-Ag14
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同种型IgG1
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab20254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
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序列相似性Belongs to the RecA family. RAD51 subfamily.
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
- Information by UniProt
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数据库链接
- Entrez Gene: 7517 Human
- Omim: 600675 Human
- SwissProt: O43542 Human
- Unigene: 592325 Human
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别名
- CMM6 antibody
- DNA repair protein XRCC3 antibody
- RAD51 like antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)](http://www.abcam.cn/ps/products/20/ab20254/Images/XRCC3-Primary-antibodies-ab20254-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)Ab20254 staining human normal placenta. Staining is localized to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
![Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)](http://www.abcam.cn/ps/products/20/ab20254/Images/XRCC3-Primary-antibodies-ab20254-2.jpg)
Western blot - Anti-XRCC3 antibody [XRCC3 10F1/6] (ab20254)
文献
This product has been referenced in:
- Li L et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014). Read more (PubMed: 24847116) »
客户评价及客户问答
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