功能Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
序列相似性Belongs to the RecA family. RAD51 subfamily.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
Immunohistochemical analysis of paraffin-embedded
human lung carcinoma tissue using ab58467 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
Western blot - XRCC3 antibody (ab58467)
All lanes : Anti-XRCC3 antibody (ab58467) at 1/500 dilution
Lane 1 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with no immunising peptide Lane 2 : extracts from HepG2 cells treated with Adriamycin (0.5µM, 5 hours) with immunising peptide
Predicted band size : 38 kDa Observed band size : 38 kDa