Anti-XRCC3抗体(ab103070)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to XRCC3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human XRCC3.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; U2OS; as well as HeLa Nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab103070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能
    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • 序列相似性
    Belongs to the RecA family. RAD51 subfamily.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

图片

  • All lanes : Anti-XRCC3 antibody (ab103070) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa
    Additional bands at: 24 kDa, 49 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab103070 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

实验方案

文献

ab103070 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Apr 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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