Anti-XRCC3抗体(ab103070)

概述

  • 产品名称Anti-XRCC3抗体
    参阅全部 XRCC3 一抗
  • 描述
    兔多克隆抗体to XRCC3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human XRCC3.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HEK293; HepG2; U2OS; as well as HeLa Nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab103070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

靶标

  • 功能Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
  • 序列相似性Belongs to the RecA family. RAD51 subfamily.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Mitochondrion. Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMM6 antibody
    • DNA repair protein XRCC3 antibody
    • RAD51 like antibody
    • X ray repair complementing defective repair in Chinese hamster antibody
    • X ray repair complementing defective repair in Chinese hamster cells 3 antibody
    • X ray repair cross complementing protein 3 antibody
    • X-ray repair cross-complementing protein 3 antibody
    • XRCC 3 antibody
    • XRCC3 antibody
    • XRCC3_HUMAN antibody
    see all

Anti-XRCC3 antibody 图像

  • All lanes : Anti-XRCC3 antibody (ab103070) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 24 kDa,49 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-XRCC3 antibody (ab103070)参考文献

ab103070 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Apr 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"