重组Anti-XRCC2抗体[EPR5149] (ab124900)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5149] to XRCC2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-XRCC2抗体[EPR5149]
参阅全部 XRCC2 一抗 -
描述
兔单克隆抗体[EPR5149] to XRCC2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human XRCC2 aa 200-300. The exact sequence is proprietary.
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阳性对照
- Human testis, HeLa, SW480, SH-SY5Y, Caco 2 and Human kidney lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5149 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124900于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). |
靶标
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功能
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. -
序列相似性
Belongs to the RecA family. RAD51 subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm, Cytoskeleton, Nucleus - Information by UniProt
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数据库链接
- Entrez Gene: 7516 Human
- Entrez Gene: 57434 Mouse
- Entrez Gene: 499966 Rat
- Omim: 600375 Human
- SwissProt: O43543 Human
- SwissProt: Q9CX47 Mouse
- Unigene: 647093 Human
- Unigene: 143767 Mouse
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别名
- DKFZp781P0919 antibody
- DNA repair protein XRCC2 antibody
- X ray repair complementing defective repair in Chinese hamster cells 2 antibody
see all
图片
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All lanes : Anti-XRCC2 antibody [EPR5149] (ab124900) at 1/10000 dilution
Lane 1 : Human testis lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : SH-SY5Y lysate
Lane 5 : Caco 2 lysate
Lane 6 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
ab124900 at 1/100 dilution staining XRCC2 in permeablised HeLa cells by intracellular flow cytometry (red). Rabbit IgG negative control antibody (green).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab124900 被引用在 1 文献中.
- Wang SP et al. FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair. Cell Death Dis 12:1138 (2021). PubMed: 34880209