Anti-XRCC1 (phospho S518 + T519 + T523)抗体(ab84417)

概述

  • 产品名称Anti-XRCC1 (phospho S518 + T519 + T523)抗体
    参阅全部 XRCC1 一抗
  • 描述
    兔多克隆抗体to XRCC1 (phospho S518 + T519 + T523)
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide to a region surrounding residues Ser 518, Thr 519 and Thr 523 of Human XRCC1, Swiss Prot P18887.

  • 阳性对照
    • Human Controls: B-cell lymphoma, breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, metastatic lymph node, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma.
      Mouse Controls: colon carcinoma CT26, squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ICC/IF
  • 应用说明ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
    • 序列相似性Contains 2 BRCT domains.
    • 翻译后修饰Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
      Sumoylated.
    • 细胞定位Nucleus. Accumulates at sites of DNA damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA repair protein XRCC1 antibody
      • RCC antibody
      • X ray repair complementing defective repair in chinese hamster antibody
      • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
      • X ray repair complementing defective repair in chinese hamster cells 1 antibody
      • X ray repair cross complementing 1 antibody
      • X ray repair cross complementing protein 1 antibody
      • X ray repair, complementing defective, repair in Chinese hamster antibody
      • X-ray repair cross-complementing protein 1 antibody
      • XRCC 1 antibody
      • Xrcc1 antibody
      • XRCC1_HUMAN antibody
      see all

    Anti-XRCC1 (phospho S518 + T519 + T523) antibody 图像

    • Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
      Samples: FFPE serial sections of Human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).
      Antibody: ab84417 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Mouse XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
      Samples: FFPE serial sections of Mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).
      Antibody: ab84417 used at a dilution of 1/250. Detection: DAB staining.
    • Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunofluorescence.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84417 used at a dilution of 1/100.
      Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.

    Anti-XRCC1 (phospho S518 + T519 + T523) antibody (ab84417)参考文献

    ab84417 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab84417.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"