Anti-XRCC1 (phospho S518 + T519 + T523)抗体(ab84417)

概述

  • 产品名称
    Anti-XRCC1 (phospho S518 + T519 + T523)抗体
    参阅全部 XRCC1 一抗
  • 描述
    兔多克隆抗体to XRCC1 (phospho S518 + T519 + T523)
  • 经测试应用
    适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide to a region surrounding residues Ser 518, Thr 519 and Thr 523 of Human XRCC1, Swiss Prot P18887.

  • 阳性对照
    • Human Controls: B-cell lymphoma, breast carcinoma, colon carcinoma, laryngeal squamous cell carcinoma, linitis plastica stomach cancer, metastatic lymph node, ovarian carcinoma, pancreatic islet cell tumor, prostate carcinoma, skin basal cell carcinoma, small cell lung cancer, stomach adenocarcinoma, testicular seminoma.
      Mouse Controls: colon carcinoma CT26, squamous cell carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab84417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
ICC/IF
  • 应用说明
    ICC/IF: 1/100.
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
    • 序列相似性
      Contains 2 BRCT domains.
    • 翻译后修饰
      Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
      Sumoylated.
    • 细胞定位
      Nucleus. Accumulates at sites of DNA damage.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA repair protein XRCC1 antibody
      • RCC antibody
      • X ray repair complementing defective repair in chinese hamster antibody
      • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
      • X ray repair complementing defective repair in chinese hamster cells 1 antibody
      • X ray repair cross complementing 1 antibody
      • X ray repair cross complementing protein 1 antibody
      • X ray repair, complementing defective, repair in Chinese hamster antibody
      • X-ray repair cross-complementing protein 1 antibody
      • XRCC 1 antibody
      • Xrcc1 antibody
      • XRCC1_HUMAN antibody
      see all

    图片

    • Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
      Samples: FFPE serial sections of Human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).
      Antibody: ab84417 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Mouse XRCC1 (phospho S518 + T519 + T523) by Immunohistochemistry.
      Samples: FFPE serial sections of Mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for XRCC1 (phospho S518 + T519 + T523).
      Antibody: ab84417 used at a dilution of 1/250. Detection: DAB staining.
    • Detection of Human XRCC1 (phospho S518 + T519 + T523) by Immunofluorescence.
      Sample: FFPE section of Human breast carcinoma.
      Antibody: ab84417 used at a dilution of 1/100.
      Detection: Red-fluorescent Alexa Fluor 555 goat anti-rabbit IgG used at a dilution of 1/500.

    文献

    This product has been referenced in:
    • Lord T & Aitken RJ Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo. Dev Biol 406:1-13 (2015). Read more (PubMed: 26234752) »

    See 1 Publication for this product

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