重组Anti-XRCC1抗体[EPR4389(2)] (ab134056)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4389(2)] to XRCC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-XRCC1抗体[EPR4389(2)]
参阅全部 XRCC1 一抗 -
描述
兔单克隆抗体[EPR4389(2)] to XRCC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human XRCC1 aa 1-100 (N terminal). The exact sequence is proprietary.
-
阳性对照
- WB: HeLa, A375, Saos-2, PC-12 and NIH/3T3 cell lysates. Mouse brain and kidney tissue lysate IHC-P: Human testis tissue. ICC/IF: HeLa, PC-12 and NIH/3T3 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4389(2) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134056于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF | (1) |
1/100 - 1/250.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 69 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
-
功能
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. -
序列相似性
Contains 2 BRCT domains. -
翻译后修饰
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated. -
细胞定位
Nucleus. Accumulates at sites of DNA damage. - Information by UniProt
-
数据库链接
- Entrez Gene: 7515 Human
- Entrez Gene: 22594 Mouse
- Entrez Gene: 84495 Rat
- Omim: 194360 Human
- SwissProt: P18887 Human
- SwissProt: Q60596 Mouse
- SwissProt: Q9ESZ0 Rat
- Unigene: 98493 Human
see all -
别名
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
see all
图片
-
All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/2000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3(Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce) at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM /TBST
Exposure time:
Lanes 1-2: 3 minutes
Lanes 3-4: 20 seconds -
Immunocytochemistry/Immunofluorescence analysis of PC-12(Rat adrenal gland pheochromocytoma) labelling with ab134056 at a dilution of 1/50 dilution (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
-
Immunocytochemistry/Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) labelling with ab134056 at a dilution of 1/50 (4.66 µg/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 dilution (2 µg/mL)) was used as the secondary antibody. The cells were co-stained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Nuclei counterstained with DAPI (blue). Control: PBS instead of the primary antibody.
-
All lanes : Anti-XRCC1 antibody [EPR4389(2)] (ab134056) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 69 kDa -
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling XRCC1 with ab134056 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab134056 staining XRCC1 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
-
Immunofluorescent analysis of HeLa cells labelling XRCC1 with ab134056 at 1/100 dilution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (16)
ab134056 被引用在 16 文献中.
- Krieger KL et al. Repair-Assisted Damage Detection Reveals Biological Disparities in Prostate Cancer between African Americans and European Americans. Cancers (Basel) 14:N/A (2022). PubMed: 35205762
- Goy E et al. The out-of-field dose in radiation therapy induces delayed tumorigenesis by senescence evasion. Elife 11:N/A (2022). PubMed: 35302491
- Jiao W et al. ZSTK474 Sensitizes Glioblastoma to Temozolomide by Blocking Homologous Recombination Repair. Biomed Res Int 2022:8568528 (2022). PubMed: 35872860
- Anurag M et al. Proteogenomic Markers of Chemotherapy Resistance and Response in Triple-Negative Breast Cancer. Cancer Discov 12:2586-2605 (2022). PubMed: 36001024
- Baquero JM et al. Small molecule inhibitor of OGG1 blocks oxidative DNA damage repair at telomeres and potentiates methotrexate anticancer effects. Sci Rep 11:3490 (2021). PubMed: 33568707