Anti-XRCC1抗体[33-2-5] (ab1838)

概述

  • 产品名称
    Anti-XRCC1抗体[33-2-5]
    参阅全部 XRCC1 一抗
  • 描述
    小鼠单克隆抗体[33-2-5] to XRCC1
  • 特异性
    This antibody is specific for XRCC1 (85 kDa).
  • 经测试应用
    适用于: WB, IP, IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human, Chinese hamster
  • 免疫原

    Recombinant full length protein corresponding to Human XRCC1.

  • 阳性对照
    • IHC-P: Human testis tissue.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab134056 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab1838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IP Use at an assay dependent concentration. PubMed: 17982170
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC/IF 1/200.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • 序列相似性
    Contains 2 BRCT domains.
  • 翻译后修饰
    Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
    Sumoylated.
  • 细胞定位
    Nucleus. Accumulates at sites of DNA damage.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA repair protein XRCC1 antibody
    • RCC antibody
    • X ray repair complementing defective repair in chinese hamster antibody
    • X ray Repair Complementing Defective Repair in Chinese Hamster Cells antibody
    • X ray repair complementing defective repair in chinese hamster cells 1 antibody
    • X ray repair cross complementing 1 antibody
    • X ray repair cross complementing protein 1 antibody
    • X ray repair, complementing defective, repair in Chinese hamster antibody
    • X-ray repair cross-complementing protein 1 antibody
    • XRCC 1 antibody
    • Xrcc1 antibody
    • XRCC1_HUMAN antibody
    see all

图片

  • All lanes : Anti-XRCC1 antibody [33-2-5] (ab1838) at 1/500 dilution

    Lane 1 : Human dermal fibroblast - whole cell lysate. Treated with non-targeting siRNA
    Lane 2 : Human dermal fibroblast - whole cell lysate. Treated with XRCC1 siRNA

    Secondary
    HRP-conjugated rabbit anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling XRCC1 with ab1838.

  • ab1838 staining XRCC1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 4% BSA for 10 minutes. Samples were incubated with primary antibody (1/200) for 1 hour. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab1838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1838, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Zhang Y  et al. Prognostic and predictive role of COX-2, XRCC1 and RASSF1 expression in patients with esophageal squamous cell carcinoma receiving radiotherapy. Oncol Lett 13:2549-2556 (2017). IHC-P ; Human . Read more (PubMed: 28454432) »
  • de Sousa MML  et al. p38 MAPK signaling and phosphorylations in the BRCT1 domain regulate XRCC1 recruitment to sites of DNA damage. Sci Rep 7:6322 (2017). Read more (PubMed: 28740101) »

See all 33 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mef)
Loading amount
40 µg
Specification
mef
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 May 16 2012

Thank you for your reply.

I am very pleased to hear you would like to accept our offer and test ab1838 in mouse. As requested, I am providing the following testing discount code:

DISCOUNT CODE: #####
Expiration date: ##### Read More

Thank you for your telephone call earlier today.

As discussed on teh phone, as far as we are aware, ab1838 has never been tested with samples from mouse]. All tested species cross-reactivity information is stated on our datasheets, and these...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (U2OS)
Loading amount
25 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Andrew Cobb

Verified customer

提交于 Mar 29 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
30 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Tris-Gly gel)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
5 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (acrylamide 9%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Dermal Fibroblast)
Loading amount
10000 cells
Specification
Dermal Fibroblast
Treatment
XRCC1 or non-targeting siRNA
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 17 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Dermal Fibroblast)
Specification
Dermal Fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Aug 28 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (cervix (Hela))
Specification
cervix (Hela)
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Alexander Rapp

Verified customer

提交于 Feb 01 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela)
Specification
Hela
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 4%
Username

Mr. Oliver Mortusewicz

Verified customer

提交于 Oct 17 2006

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