概述

  • 产品名称Anti-XPF抗体
    参阅全部 XPF 一抗
  • 描述
    兔多克隆抗体to XPF
  • 经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residues 855 and 905 of human XPF (AAB07689.1)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab76948 was affinity purified using an epitope specific to XPF immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab76948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 103 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
  • 疾病相关Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
  • 序列相似性Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA excision repair protein ERCC 4 antibody
    • DNA excision repair protein ERCC-4 antibody
    • DNA excision repair protein ERCC4 antibody
    • DNA repair endonuclease XPF antibody
    • DNA repair protein complementing XP F cells antibody
    • DNA repair protein complementing XP-F cells antibody
    • ERCC 11 antibody
    • ERCC 4 antibody
    • ERCC11 antibody
    • ERCC4 antibody
    • Excision repair complementing defective in Chinese hamster antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
    • excision repair cross-complementation group 4 antibody
    • FANCQ antibody
    • RAD 1 antibody
    • RAD1 antibody
    • Xeroderma pigmentosum complementation group F antibody
    • Xeroderma pigmentosum group F complementing protein antibody
    • Xeroderma pigmentosum group F-complementing protein antibody
    • Xeroderma pigmentosum VI antibody
    • XP, group G antibody
    • XP6 antibody
    • XPF_HUMAN antibody
    see all

Anti-XPF antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-XPF antibody (ab76948) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 103 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
  • ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
  • ICC/IF image of ab76948 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76948, 1µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-XPF antibody (ab76948)参考文献

This product has been referenced in:
  • Cavallo F  et al. Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition. PLoS One 7:e51563 (2012). WB ; Mouse . Read more (PubMed: 23251575) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (6%)
Sample Human Cell lysate - whole cell (HeLa cell line)
Specification HeLa cell line
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Dec 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"