- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-XPF抗体
参阅全部 XPF 一抗 -
描述兔多克隆抗体to XPF
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宿主Rabbit
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经测试应用适用于: WB, IP, ICC/IF, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to a region between residues 855 and 905 of human XPF (AAB07689.1)
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阳性对照
- HeLa and 293T whole cell lysates.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab76948 was affinity purified using an epitope specific to XPF immobilized on solid support.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab76948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 103 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
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疾病相关Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly. -
序列相似性Belongs to the XPF family.
Contains 1 ERCC4 domain. -
细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2072 Human
- Omim: 278760 Human
- SwissProt: Q92889 Human
- Unigene: 567265 Human
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别名
- DNA excision repair protein ERCC 4 antibody
- DNA excision repair protein ERCC-4 antibody
- DNA excision repair protein ERCC4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XPF antibody (ab76948)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling XPF with ab76948 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-XPF antibody (ab76948)All lanes : Anti-XPF antibody (ab76948) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 103 kDa
Observed band size: 117 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - Anti-XPF antibody (ab76948)ab76948 at 1µg/ml staining XPF in HeLa whole cell lysate immunoprecipitated using ab76948 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). -
Immunocytochemistry/ Immunofluorescence - Anti-XPF antibody (ab76948)ICC/IF image of ab76948 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76948, 1µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
文献
This product has been referenced in:
- Popp I et al. Fanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF. BMC Med Genet 19:7 (2018). Read more (PubMed: 29325523) »
- Cavallo F et al. Reduced proficiency in homologous recombination underlies the high sensitivity of embryonal carcinoma testicular germ cell tumors to Cisplatin and poly (adp-ribose) polymerase inhibition. PLoS One 7:e51563 (2012). WB ; Mouse . Read more (PubMed: 23251575) »
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