概述

  • 产品名称Anti-XPF抗体
    参阅全部 XPF 一抗
  • 描述
    兔多克隆抗体to XPF
  • 经测试应用适用于: WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human XPF.

  • 阳性对照
    • Extracts from 293 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.
ELISA 1/10000.
IP Use at an assay dependent concentration. PubMed: 24726326

靶标

  • 功能Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
  • 疾病相关Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.
    Defects in ERCC4 are a cause of XFE progeroid syndrome (XFEPS) [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.
  • 序列相似性Belongs to the XPF family.
    Contains 1 ERCC4 domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA excision repair protein ERCC 4 antibody
    • DNA excision repair protein ERCC-4 antibody
    • DNA excision repair protein ERCC4 antibody
    • DNA repair endonuclease XPF antibody
    • DNA repair protein complementing XP F cells antibody
    • DNA repair protein complementing XP-F cells antibody
    • ERCC 11 antibody
    • ERCC 4 antibody
    • ERCC11 antibody
    • ERCC4 antibody
    • Excision repair complementing defective in Chinese hamster antibody
    • Excision repair cross complementing rodent repair deficiency complementation group 4 antibody
    • excision repair cross-complementation group 4 antibody
    • FANCQ antibody
    • RAD 1 antibody
    • RAD1 antibody
    • Xeroderma pigmentosum complementation group F antibody
    • Xeroderma pigmentosum group F complementing protein antibody
    • Xeroderma pigmentosum group F-complementing protein antibody
    • Xeroderma pigmentosum VI antibody
    • XP, group G antibody
    • XP6 antibody
    • XPF_HUMAN antibody
    see all

Anti-XPF antibody 图像

  • All lanes : Anti-XPF antibody (ab73720) at 1/500 dilution

    Lane 1 : Extracts from 293 cells
    Lane 2 : Extracts from 293 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 103 kDa
    Observed band size : 103 kDa

Anti-XPF antibody (ab73720)参考文献

This product has been referenced in:
  • Hodskinson MR  et al. Mouse SLX4 is a tumor suppressor that stimulates the activity of the nuclease XPF-ERCC1 in DNA crosslink repair. Mol Cell 54:472-84 (2014). WB, IP ; Mouse . Read more (PubMed: 24726326) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount 100 µg
Specification GM00637 fibroblast cell line
Gel Running Conditions Reduced Denaturing (10%)
Blocking step (agent) for 30 minute(s) · Concentration: 5µg/mL · Temperature: 20°C
Username

Mrs. Annika Schäfer

Verified customer

提交于 Apr 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"