Anti-XPB抗体(ab99322)
Key features and details
- Rabbit polyclonal to XPB
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-XPB抗体
参阅全部 XPB 一抗 -
描述
兔多克隆抗体to XPB -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish -
免疫原
Synthetic peptide, corresponding to a region within amino acids 575-625 of Human XPB (NP_000113.1).
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阳性对照
- HeLa whole cell lysate (ab150035)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
ATP-dependent 3'-5' DNA helicase, component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. -
疾病相关
Defects in ERCC3 are the cause of xeroderma pigmentosum complementation group B (XP-B) [MIM:610651]; also known as xeroderma pigmentosum II (XP2) or XP group B (XPB) or xeroderma pigmentosum group B combined with Cockayne syndrome (XP-B/CS). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-B patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC3 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP. -
序列相似性
Belongs to the helicase family. RAD25/XPB subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 507984 Cow
- Entrez Gene: 2071 Human
- Entrez Gene: 13872 Mouse
- Entrez Gene: 100172725 Orangutan
- Entrez Gene: 100348395 Rabbit
- Entrez Gene: 291703 Rat
- Entrez Gene: 324323 Zebrafish
- Omim: 133510 Human
see all -
别名
- Basic transcription factor 2 89 kDa subunit antibody
- BTF 2 antibody
- BTF2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling XPB with ab99322 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of XPB in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99322 at 3 µg/mg lysate for IP. An anti-XPB antibody, which recognizes a downstream epitope was used at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab99322 被引用在 1 文献中.
- Wang S et al. Overexpression of ERCC3 is associated with poor prognosis in patients with pancreatic cancer. J Cancer 12:2550-2559 (2021). PubMed: 33854616