概述

  • 产品名称
  • 描述
    兔多克隆抗体to XPA
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rhesus monkey, Gorilla, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 1 and 50 of human XPA (NP_000371.1).

  • 阳性对照
    • Human controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma Mouse controls: Teratoma

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab84780 was affinity purified using an epitope specific to XPA immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
    • 组织特异性
      Expressed in various cell lines and in skin fibroblasts.
    • 疾病相关
      Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
    • 序列相似性
      Belongs to the XPA family.
    • 翻译后修饰
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      Ubiquitinated by HERC2 leading to degradation by the proteasome.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DNA repair protein complementing XP A cells antibody
      • DNA repair protein complementing XP-A cells antibody
      • DNA repair protein complementing XPA cells antibody
      • Excision repair controlling antibody
      • Xeroderma pigmentosum 1 antibody
      • Xeroderma pigmentosum complementation group A antibody
      • Xeroderma pigmentosum group A complementing protein antibody
      • Xeroderma pigmentosum group A-complementing protein antibody
      • XP 1 antibody
      • XP1 antibody
      • xpa antibody
      • XPA_HUMAN antibody
      • Xpac antibody
      see all

    图片

    • ab84780, at a 1/500 dilution, staining XPA in formalin fixed, paraffin embedded human ovarian tumor by Immunohistochemistry using DAB staining.
    • ab84780, at a 1/500 dilution, staining XPA in formalin fixed, paraffin embedded human colon carcinoma by Immunohistochemistry using DAB staining.
    • ab84780, at a 1/100 dilution, staining XPA in formalin fixed, paraffin embedded mouse teratoma by Immunohistochemistry using DAB staining.

    文献

    ab84780 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Adult skin)
    Specification
    Adult skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ciitric buffer, pH 6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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