Anti-XLF抗体- Carboxyterminal end (ab53537)


  • 产品名称Anti-XLF抗体- Carboxyterminal end
    参阅全部 XLF 一抗
  • 描述
    山羊多克隆抗体to XLF - Carboxyterminal end
  • 经测试应用适用于: WB, ELISA, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide:


    , corresponding to C terminal amino acids 282-295 of Human XLF

  • 阳性对照
    • Human Skin, Testis and Thyroid gland lysates


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab53537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
ELISA 1/64000. Peptide ELISA
ICC Use a concentration of 2.5 - 3.8 µg/ml.


  • 功能DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
    Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
  • 序列相似性Belongs to the XLF family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cernunnos antibody
    • FLJ12610 antibody
    • NHEJ 1 antibody
    • Nhej1 antibody
    • NHEJ1, S. cerevisiae, homolog of antibody
    • NHEJ1_HUMAN antibody
    • Non homologous end joining factor 1 antibody
    • Non-homologous end-joining factor 1 antibody
    • Nonhomologous end joining factor 1 antibody
    • OTTHUMP00000164168 antibody
    • OTTHUMP00000206275 antibody
    • OTTHUMP00000206279 antibody
    • Protein cernunnos antibody
    • XLF antibody
    • XRCC4 like factor antibody
    • XRCC4-like factor antibody
    see all

Anti-XLF antibody - Carboxyterminal end 图像

  • ab53537 antibody (0.1µg/ml) staining of XLF in Human Thyroid lysate (35µg protein in RIPA buffer)

    Predicted band size : 33 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • ab53537 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-XLF antibody - Carboxyterminal end (ab53537)参考文献

ab53537 has not yet been referenced specifically in any publications.

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