概述

  • 产品名称Anti-XLF抗体[1F3]
    参阅全部 XLF 一抗
  • 描述
    小鼠单克隆抗体[1F3] to XLF
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human XLF.

  • 阳性对照
    • HEK293T cellstransfected with pCMV6-ENTRY XLF cDNA, Human breast, Human Kidney, Adenocarcinoma of Human ovary, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate and Carcinoma of Human bladder tissues, COS7 cells transiently transfected with XLF
  • 常规说明Dilute in PBS (pH7.3) before use.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号1F3
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 33 kDa.
IHC-P 1/150.
ICC/IF 1/100.

靶标

  • 功能DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in NHEJ1 are the cause of severe combined immunodeficiency due to NHEJ1 deficiency (NHEJ1-SCID) [MIM:611291]; also known as autosomal recessive T cell-negative, B cell-negative, NK cell-positive, severe combined immunodeficiency with microcephaly, growth retardation and sensitivity to ionizing radiation or NHEJ1 syndrome. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. NHEJ1-SCID is characterized by a profound T- and B-lymphocytopenia associated with increased cellular sensitivity to ionizing radiation, microcephaly and growth retardation. Some patients may manifest SCID with sensitivity to ionizing radiation without microcephaly and mild growth retardation, probably due to hypomorphic NHEJ1 mutations.
    Note=A chromosomal aberration involving NHEJ1 is found in a patient with polymicrogyria. Translocation t(2;7)(q35;p22).
  • 序列相似性Belongs to the XLF family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cernunnos antibody
    • FLJ12610 antibody
    • NHEJ 1 antibody
    • Nhej1 antibody
    • NHEJ1, S. cerevisiae, homolog of antibody
    • NHEJ1_HUMAN antibody
    • Non homologous end joining factor 1 antibody
    • Non-homologous end-joining factor 1 antibody
    • Nonhomologous end joining factor 1 antibody
    • OTTHUMP00000164168 antibody
    • OTTHUMP00000206275 antibody
    • OTTHUMP00000206279 antibody
    • Protein cernunnos antibody
    • XLF antibody
    • XRCC4 like factor antibody
    • XRCC4-like factor antibody
    see all

Anti-XLF antibody [1F3] 图像



  • Predicted band size : 33 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: XLF knockout HAP1 cell lysate (20 µg)

    Lanes 1 - 2: Merged signal (red and green). Green – ab119375 observed at 38 kDa. Red - loading control, ab129002, observed at 124 kDa.

    ab119375 was shown to specifically react with XLF when XLF knockout samples were used. Wild-type and XLF knockout samples were subjected to SDS-PAGE. ab119375 and ab129002 (loading control to Vinculin) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-XLF antibody [1F3] (ab119375) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY XLF cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 33 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203393)
  • Immunohistochemical staining of paraffin-embedded Human breast tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Human prostate tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using ab119375 at a dilution of 1/150.
  • Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using ab119375 at a dilution of 1/150.
  • Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF using ab119375 at a dilution of 1/100.

Anti-XLF antibody [1F3] (ab119375)参考文献

ab119375 has not yet been referenced specifically in any publications.

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