XIAP overexpression 293T裂解液(全细胞) (ab94239)

概述

  • 产品名称XIAP overexpression 293T裂解液(全细胞)
  • 描述
    XIAP overexpression裂解液
  • 常规说明ab94239 is a 293T cell transfected lysate in which Human XIAP has been transiently over-expressed using a pCMV-XIAP plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. Tissue specificity: Ubiquitous, except peripheral blood leukocytes. Disease: Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. Similarity: Belongs to the IAP family. Contains 3 BIR repeats. Contains 1 RING-type zinc finger. Domain: The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. PTM: Ubiquitinated and degraded by the proteasome in apoptotic cells. Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.

应用

Our Abpromise guarantee covers the use of ab94239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

XIAP overexpression 293T lysate (whole cell) 图像

  • ab94239 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-XIAP antibody (ab54788) at 1/500 dilution

    Lane 1 : XIAP overexpression 293T lysate (whole cell) (ab94239)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

XIAP overexpression 293T lysate (whole cell) (ab94239)参考文献

ab94239 has not yet been referenced specifically in any publications.

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