XIAP overexpression 293T裂解物(whole cell) (ab94239)
概述
-
产品名称
XIAP overexpression 293T裂解物(whole cell)
参阅全部 XIAP 细胞裂解液 -
常规说明
ab94239 is a 293T cell transfected lysate in which Human XIAP has been transiently over-expressed using a pCMV-XIAP plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
-
经测试应用
适用于: WBmore details
性能
-
Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究领域
-
背景
Function: Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. Tissue specificity: Ubiquitous, except peripheral blood leukocytes. Disease: Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. Similarity: Belongs to the IAP family. Contains 3 BIR repeats. Contains 1 RING-type zinc finger. Domain: The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. PTM: Ubiquitinated and degraded by the proteasome in apoptotic cells. Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
说明 |
---|
WB
Use at an assay dependent dilution. |
图片
-
ab94239 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-XIAP antibody (ab54788) at 1/500 dilution
Lane 1 :XIAP overexpression 293T lysate (whole cell) (ab94239)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab94239 尚未被引用在任何文献中。