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Our Abpromise guarantee covers the use of ab28151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22039248|
ab28151 staining XIAP in Mouse colonic epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 1% BSA for 45 minutes at 20°C. Samples were incubated with primary antibody (1/50 in 1% BSA + 0.01% Triton X-100) for 2 hours at 22°C. An Alexa Fluor® 488-conjugated Chicken anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"