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Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT
Our Abpromise guarantee covers the use of ab37185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.
xCT forms heterodimer with 4F2Hc protein hence reducing conditions should be used in WB. It exists as multi-pass membrane protein therefore we recommend heating the lysates for 15-20 minutes at 60-70C.
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 100 - 1000 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 21639880|