概述

  • 产品名称Anti-xCT抗体
    参阅全部 xCT 一抗
  • 描述
    兔多克隆抗体to xCT
  • 特异性This antibody is specific for XCT.
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide: MVRKPVVSTI SKGGYLQGNV NGRLPSLGNK EPPGQEKVQL , corresponding to N terminal amino acids 1/50 of Human xCT

  • 阳性对照
    • human and mouse Stomach tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab37185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.

xCT forms heterodimer with 4F2Hc protein hence reducing conditions should be used in WB. It exists as multi-pass membrane protein therefore we recommend heating the lysates for 15-20 minutes at 60-70C.

Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 100 - 1000 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21639880

靶标

  • 功能Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • 序列相似性Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

Anti-xCT antibody 图像

  • All lanes : Anti-xCT antibody (ab37185) at 0.5 µg/ml

    Lane 1 : Total human stomach lysate.
    Lane 2 : Total mouse stomach lysate.

    Developed using the ECL technique

    Predicted band size : 55 kDa
    Observed band size : 55 kDa


    Exposure time : 1 minute

Anti-xCT antibody (ab37185)参考文献

This product has been referenced in:
  • Evonuk KS  et al. Inhibition of System Xc(-) Transporter Attenuates Autoimmune Inflammatory Demyelination. J Immunol 195:450-63 (2015). Read more (PubMed: 26071560) »
  • Sha LK  et al. Loss of Nrf2 in bone marrow-derived macrophages impairs antigen-driven CD8(+) T cell function by limiting GSH and Cys availability. Free Radic Biol Med 83:77-88 (2015). Flow Cyt ; Mouse . Read more (PubMed: 25687825) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Loading amount 20 µg
Treatment drug 48h
Specification head and neck cancer
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 21 2015

Application IHC - Wholemount
Sample Human Tissue (head and neck cancer)
Specification head and neck cancer
Username

Miss. jin young park

Verified customer

提交于 Oct 19 2015

Thanks so muchfor your reply and best of luck with your upcoming experiments. If you have any further questions please do not hesitate to contact me.

Thanks for submitting your enquiry. I am sorry to hear this antibody is giving you trouble.

I was just wondering what positive control samples you have used?

Thanks in advance for the additional information.

Thank you for contacting us and making us aware of this. We have been transitioning to a new email system and it may be that the response which I had sent on Mon 16 Apr 12 13:26GMT was somehow caught up or perhaps it went directly to your spam or ju...

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Thank you for your response and for the additional details which you have provided.

You are correct as this antibody will only recognize the intracellular epitope of xCT. As such you would need to permeablize your cells to allow entry of the...

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Thank you for contacting Abcam, I am sorry to hear that you have been experiencing difficulties using this product in flow.

Would you be able to provide me the specific details of your experiments? Useful information would include but not be...

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Thank you for contacting Abcam.

We are unable to send you any free/trail sizes of our antibodies. When your ordering system is reorganized at the end of the month, you will be able to order the antibody and if it is in stock then it will be s...

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Thank you for your call today and for letting us know about the trouble with ab37185. As we discussed, please let me know if the suggestions that we discussed don't improve the results, and I will be happy to help you further. If you have any questions...

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Thank you for your enquiry. As discussed on the phone, here is the email that was sent in response to your questionnaire from Kate Hayes on December 1st, 2006. Reply from Abcam to your enquiry regarding ab37185 Dear Ilangovan, In response to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"