概述

  • 产品名称
  • 描述
    山羊多克隆抗体to xCT
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    KGQTQNFKDAFSGRD

    by a Cysteine residue linker, corresponding to internal sequence amino acids 215-229 of Human xCT.

  • 阳性对照
    • Human Kidney and Placenta tissues; Human Spleen lysate.

性能

应用

Our Abpromise guarantee covers the use of ab111822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/128000.
WB Use a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 55 kDa.
IHC-P Use a concentration of 3.75 µg/ml.

靶标

  • 功能
    Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate.
  • 序列相似性
    Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amino acid transport system xc xCT antibody antibody
    • Amino acid transport system xc- antibody
    • Calcium channel blocker resistance protein CCBR1 antibody
    • Calcium channel blocker resistance protein CCBR1 antibody antibody
    • CCBR1 antibody
    • Cysteine/glutamate transporter antibody antibody
    • Cystine/glutamate transporter antibody
    • OTTHUMP00000164578 antibody
    • SLC7A11 antibody
    • Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody
    • solute carrier family 7 antibody
    • Solute carrier family 7 member 11 antibody
    • Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody
    • SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody
    • xCT antibody
    • XCT_HUMAN antibody
    see all

图片

  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue by Immunohistochemistry.
  • ab111822 at 3.75 µg/ml staining xCT in Formalin-Fixed, Paraffin-Embedded Human Placenta tissue by Immunohistochemistry.
  • Anti-xCT antibody (ab111822) at 0.02 µg/ml (for one hour) + Human Spleen lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 55 kDa

文献

This product has been referenced in:
  • Ju HQ  et al. Redox Regulation of Stem-like Cells Though the CD44v-xCT Axis in Colorectal Cancer: Mechanisms and Therapeutic Implications. Theranostics 6:1160-75 (2016). Read more (PubMed: 27279909) »

See 1 Publication for this product

客户评价及客户问答

We have added product ab178004, xCT peptide, as a blocking peptide to be used with ab111822. Here is a link: http://www.abcam.com/xct-peptide-ab178004.html.

Application
Flow Cytometry
Sample
Human Cell (liver hepatocellular cells HepG2)
Specification
liver hepatocellular cells HepG2
Preparation
Cell harvesting/tissue preparation method: trypsinized and resuspended in PBS+1% serum+0.1% NaN3
Sample buffer: PBS+1% serum+0.1% NaN3
Fixation
did not fix
Permeabilization
No
Gating Strategy
ungated cell population
Username

Yuzhe

Verified customer

提交于 Sep 05 2012

Thank you for contacting us. You are correct, ab111822 is raised against an extracellular region of xCT. As such you should not have to permeablize your cells to use this product. I hope that this information is helpful. Please let me know if y...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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