Anti-XBP1抗体(ab85546)

概述

  • 产品名称
  • 描述
    山羊多克隆抗体to XBP1
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Mouse, Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide:

    NHSWEDTFANELFPQ

    by a Cysteine residue linker, corresponding to C terminal amino acids 358-372 of Human XBP1 isoform 2

  • 阳性对照
    • Human breast tissue; HeLa nuclear lysate

性能

应用

Our Abpromise guarantee covers the use of ab85546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 40 kDa.
ELISA 1/4000.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
  • 疾病相关
    Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
  • 序列相似性
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Tax responsive element binding protein 5 antibody
    • Tax-responsive element-binding protein 5 antibody
    • TREB5 antibody
    • X box binding protein 1 antibody
    • X box binding protein 2 antibody
    • X-box-binding protein 1 antibody
    • XBP 1 antibody
    • XBP-1 antibody
    • XBP1 antibody
    • XBP1_HUMAN antibody
    • XBP2 antibody
    see all

Anti-XBP1 antibody 图像

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded human breast tissue using ab85546 at a dilution of 5µg/ml.
  • Anti-XBP1 antibody (ab85546) at 1 µg/ml + nuclear HeLa lysate in RIPA buffer at 35 µg

    Predicted band size : 40 kDa

Anti-XBP1 antibody (ab85546)参考文献

ab85546 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat-buffer
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

提交于 Dec 11 2015

Thank you very much for your call yesterday and for forwarding this dog sequence to me.

Looking at the alignment results, I do think it is quite possible for ab37152 to cross-react with your dog samples. I'm sending your information to Cedarl...

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Thank you for your call today and for your interest in our XBP1 antibodies. Please see below for information about your testing code, and let me know if you have any questions or if there is anything else that we can do for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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