重组Anti-Xanthine Oxidase抗体[EPR4605] (ab109235)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4605] to Xanthine Oxidase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Xanthine Oxidase抗体[EPR4605]
参阅全部 Xanthine Oxidase 一抗 -
描述
兔单克隆抗体[EPR4605] to Xanthine Oxidase -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal liver, Mouse and Rat liver tissue lysates, 293T cell lysates, Human milk IHC-P: Human liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4605 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109235于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa).
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 146 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
靶标
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功能
Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). -
组织特异性
Detected in milk (at protein level). -
疾病相关
Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. -
序列相似性
Belongs to the xanthine dehydrogenase family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 FAD-binding PCMH-type domain. -
翻译后修饰
Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). -
细胞定位
Cytoplasm. Peroxisome. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7498 Human
- Entrez Gene: 22436 Mouse
- Entrez Gene: 497811 Rat
- Omim: 607633 Human
- SwissProt: P47989 Human
- SwissProt: Q00519 Mouse
- SwissProt: P22985 Rat
- Unigene: 250 Human
see all -
别名
- Xanthine dehydrogenase antibody
- Xanthine dehydrogenase/oxidase antibody
- Xanthine oxidase antibody
see all
图片
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All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/10000 dilution (Purified)
Lane 1 : Mouse liver lysates
Lane 2 : Rat liver lysates
Lane 3 : Mouse heart lysates
Lane 4 : Mouse brain lysates
Lane 5 : Mouse lung lysates
Lane 6 : Mouse kidney lysates
Lane 7 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDa
Observed band size: 146 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
Xanthine Oxidase is abundantly expressed in liver and low in heart, brain and lung as what is described in PMID: 10462034
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Xanthine Oxidase with purified ab109235 at 1/100 dilution (2.3 µg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/5000 dilution (Purified) + Human milk lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 146 kDa
Observed band size: 146 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal liver lysate
Lane 2 : 293T lysate
Lane 3 : Human milk lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 146 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunohistochemical analysis of Xanthine Oxidase expression in paraffin-embedded Human liver tissue using ab109235 (unpurified) at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab109235 被引用在 24 文献中.
- Hayakawa Y et al. Vidarabine, an anti-herpes agent, prevents occlusal-disharmony-induced cardiac dysfunction in mice. J Physiol Sci 72:2 (2022). PubMed: 35148678
- Sun Z et al. Anti-Gouty Arthritis and Anti-Hyperuricemia Properties of Sanghuangporus vaninii and Inonotus hispidus in Rodent Models. Nutrients 14:N/A (2022). PubMed: 36297105
- Tang DH et al. Inosine induces acute hyperuricaemia in rhesus monkey (Macaca mulatta) as a potential disease animal model. Pharm Biol 59:175-182 (2021). PubMed: 33715593
- Zhou H et al. ASMq protects against early burn wound progression in rats by alleviating oxidative stress and secondary mitochondria-associated apoptosis via the Erk/p90RSK/Bad pathway. Mol Med Rep 23:N/A (2021). PubMed: 33760179
- Horvath C et al. Inhibition of Cardiac RIP3 Mitigates Early Reperfusion Injury and Calcium-Induced Mitochondrial Swelling without Altering Necroptotic Signalling. Int J Mol Sci 22:N/A (2021). PubMed: 34360749