The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
Use at an assay dependent concentration.
1/100000 - 1/400000.
Use a concentration of 2.5 µg/ml.
功能Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
组织特异性Detected in milk (at protein level).
疾病相关Defects in XDH are the cause of xanthinuria type 1 (XU1) [MIM:278300]. Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol.
序列相似性Belongs to the xanthine dehydrogenase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding PCMH-type domain.
翻译后修饰Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).
ab85541 at 2.5µg/ml staining Xanthine Oxidase in Formalin-Fixed Paraffin-Embedded human liver tissue. A after heat induced antigen retrieval step was performed using pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Anti-Xanthine Oxidase antibody (ab85541)参考文献
has not yet been referenced specifically in any publications.