Xanthine Oxidase Activity Assay试剂盒(ab102522)

概述

  • 产品名称
    Xanthine Oxidase Activity Assay试剂盒
  • 样品类型
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型
    Enzyme activity
  • 灵敏度
    > 10 mU/ml
  • 范围
    10 mU/ml - 1000 mU/ml
  • 检测时间
    0h 40m
  • 产品概述

    Xanthine Oxidase Activity Assay Kit (ab102522) is an easy and sensitive assay to determine xanthine oxidase (XO) activity in variety of samples. In the assay, XO present in the sample oxidizes xanthine to hydrogen peroxide (H2O2) which reacts stoichiometrically with OxiRed Probe to generate color (at OD = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to XO content, the XO activity can be accurately measured. The kit detects 1-100 mU xanthine oxidase in 100 µl reaction volume.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Xanthine oxidase (XO, EC 1.17.3.2 ) is present in appreciable amounts in the liver and jejunum in healthy individuals. However, in various liver disorders, XO is released into circulation. Therefore, determination of serum XO level serves as a sensitive indicator of acute liver damage such as jaundice.

  • 经测试应用
    适用于: Functional Studiesmore details

性能

应用

Our Abpromise guarantee covers the use of ab102522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

图片

  • Xanthine Oxidase Positive Control

实验方案

文献

This product has been referenced in:
  • Li J  et al. Artemisinins Target GABAA Receptor Signaling and Impair a Cell Identity. Cell 168:86-100.e15 (2017). Functional Studies . Read more (PubMed: 27916275) »
  • Hwang KA  et al. Antioxidant activities and oxidative stress inhibitory effects of ethanol extracts from Cornus officinalis on raw 264.7 cells. BMC Complement Altern Med 16:196 (2016). Read more (PubMed: 27391600) »

See all 3 Publications for this product

客户评价及客户问答

It will be possible to prepare and use the ab102522 Xanthine Oxidase Assay Kit several times over two months.

Once you have opened the kit and prepared the components, it is highly recommended that the components be stored in aliquots at -20C....

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"Cell culture supernatants" in this instance means cell lysates. Xanthine oxidase is not released by healthy cells. To prepare cell lysates, simply pellet the cells, estimate the volume of the pellet, and add 4 volumes of the assay buffer that is provi...

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I can confirm that the kit ab102522 and ab102500 can be used to assay xanthine oxidase and Hydrogen peroxide in cells. I would suggest starting with at least 1-2 million cells. Please follow the following protocol;

2X106 cells, suspend the ce...

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En cuanto al principio de la reacción, en el “enzyme mix” no hay enzima XO. Este reactivo actúa en el segundo paso de la reacción, después de que la enzima XO de las muestras haya degradado la Xantina. El en...

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The cell pellet after removing all media can be stored at -80C with a small volume of PBS. It is not ideal to store cells with medium. Once thawed, the cells can be washed once with PBS and then the PBS can be removed. At this step the assay buffer can...

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I have double checked with the lab. Yes, the assay buffer is sufficient for efficient homogenization. You don’t need any other buffer. Just look at an aliquot of the homogenised sample under the microscope. If most cells show a broken nuclear ri...

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Thank you for your enquiry.

I am pleased to provide the following information for you:

1. Is there a preferred method to lift up the cells before centrifuging to get the supernatant/xanthine oxidase?

Cells can be trypsi...

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Thanks for your inquiry.
You can most likely use frozen tissues with limitations. We ourselves have never tried it, but I think if the tissues have been frozen efficiently and stored well, they should not pose a problem. Unfortunately we cannot gu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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