Anti-XAF1抗体(ab81353)

概述

  • 产品名称Anti-XAF1抗体
    参阅全部 XAF1 一抗
  • 描述
    兔多克隆抗体to XAF1
  • 特异性No cross reactivity with other proteins.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of human XAF1, identical to the related rat and mouse sequence.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab81353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with XAF1 peptide (ab110792).
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
  • 组织特异性Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
  • 序列相似性Contains 1 TRAF-type zinc finger.
  • 发展阶段Selectively expressed in third trimester placenta.
  • 细胞定位Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BIRC 4 binding protein antibody
    • BIRC4 binding protein antibody
    • BIRC4-binding protein antibody
    • BIRC4BP antibody
    • HSXIAPAF 1 antibody
    • HSXIAPAF1 antibody
    • XAF 1 antibody
    • XAF1 antibody
    • XAF1_HUMAN antibody
    • XIAP associated factor 1 antibody
    • XIAP-associated factor 1 antibody
    • XIAPAF1 antibody
    see all

Anti-XAF1 antibody 图像

  • All lanes : Anti-XAF1 antibody (ab81353) at 1 µg/ml

    Lane 1 : Tissue lysates prepared from Rat liver tissue.
    Lane 2 : Tissue lysates prepared from Rat kidney tissue.
    Lane 3 : Tissue lysates prepared from Rat spleen tissue.
    Lane 4 : Tissue lysates prepared from Human placenta tissue lysates.
    Lane 5 : Molecular marker
    Lane 6 : Whole cell lysates prepared from MCF7 cells.
    Lane 7 : Whole cell lysates prepared from Hela cells.
    Lane 8 : Whole cell lysates prepared from SMMC cells.
    Lane 9 : Whole cell lysates prepared from HT1080 cells.
    Lane 10 : Whole cell lysates prepared from colo320 cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 35 kDa
  • ab81353 at 1µg/ml staining XAF1 in Human endometrium cancer tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded sections). The tissue sections underwent antigen retrieval by heat. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

  • ICC/IF image of ab81353 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81353, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-XAF1 antibody (ab81353)参考文献

ab81353 has not yet been referenced specifically in any publications.

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