概述

  • 产品名称Anti-XAF1抗体
    参阅全部 XAF1 一抗
  • 描述
    兔多克隆抗体to XAF1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).

  • 阳性对照
    • Human spleen cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克��
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab17204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18485100
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with Human XAF1 peptide (ab39906). A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
  • 组织特异性Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
  • 序列相似性Contains 1 TRAF-type zinc finger.
  • 发展阶段Selectively expressed in third trimester placenta.
  • 细胞定位Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BIRC 4 binding protein antibody
    • BIRC4 binding protein antibody
    • BIRC4-binding protein antibody
    • BIRC4BP antibody
    • HSXIAPAF 1 antibody
    • HSXIAPAF1 antibody
    • XAF 1 antibody
    • XAF1 antibody
    • XAF1_HUMAN antibody
    • XIAP associated factor 1 antibody
    • XIAP-associated factor 1 antibody
    • XIAPAF1 antibody
    see all

Anti-XAF1 antibody 图像

  • Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
    Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
    Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml

    Lane 1 : Human spleen lysate
    Lane 2 : Human spleen lysate
    Lane 3 : Human spleen lysate


    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen.
  • ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC

  • ICC/IF image of ab17204 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-XAF1 antibody (ab17204)参考文献

This product has been referenced in:
  • Chen ZX  et al. RNA helicase A is a downstream mediator of KIF1Bß tumor-suppressor function in neuroblastoma. Cancer Discov 4:434-51 (2014). Read more (PubMed: 24469107) »
  • Qiu W  et al. Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation. Cell Death Dis 5:e1176 (2014). Read more (PubMed: 24743731) »

See all 5 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 24 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Dec 22 2014

Thank you for your enquiry. I can confirm the following antigen retrieval methods were used in testing of these antibodies. Please not that this may require individual optimization by customers in their own experiments: ab17204 XAF1 antibody ...

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Thank you for calling Abcam earlier. Please let me know how you would like to proceed in regards to testing a second XAF1 antibody, potentially ab98913, which is the antibody we talked about on the telephone. I look forward to your reply

Thank you for your email. Since the antibody was raised against the C-terminal region of the full length protein, the antibody can bind to the following isoforms: isoform 1 (35 kDa) isoform 2 (32 kDa) isoform 6 (22.5 kDa) isoform 7 (34.5 kDa) ...

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Thank you for your enquiry. According to the following publication XIAP is localised to the nucleus in HeLa cells transfected with an RFP tagged version of the protein. Identification of XAF1 as an antagonist of XIAP anti-Caspase activity. 2001 ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - other (primary cortical neurons)
Loading amount 25 µg
Specification primary cortical neurons
Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1x
Username

Abcam user community

Verified customer

提交于 May 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"