Anti-XAF1抗体(ab17204)
Key features and details
- Rabbit polyclonal to XAF1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-XAF1抗体
参阅全部 XAF1 一抗 -
描述
兔多克隆抗体to XAF1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17204于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 18485100
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 18485100 |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. -
组织特异性
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). -
序列相似性
Contains 1 TRAF-type zinc finger. -
发展阶段
Selectively expressed in third trimester placenta. -
细胞定位
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 54739 Human
- Entrez Gene: 327959 Mouse
- Entrez Gene: 679600 Rat
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- SwissProt: Q5NBU8 Mouse
- Unigene: 441975 Human
- Unigene: 291131 Mouse
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别名
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
see all
图片
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Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lanes 2-3 : Human spleen lysate
Observed band size: 32 kDa why is the actual band size different from the predicted?Western blot analysis of XAF1 in human spleen lysate with ab17204 at
Lane A: 0.5 ug/ml
Lane B: 1ug/ml
Lane C: 2ug/ml
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen. -
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab17204 被引用在 13 文献中.
- Sun G et al. WZY-321 triggers glioma cell apoptosis via XAF1 up-regulation caused by MTM-mediated miR-873 down-regulation. J Cancer 13:2312-2321 (2022). PubMed: 35517406
- Zhu C et al. SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. J Exp Med 216:743-756 (2019). PubMed: 30842236
- Xu T et al. Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Mol Ther Nucleic Acids 17:405-413 (2019). PubMed: 31310946
- Jiang X et al. Bisphenol A induced male germ cell apoptosis via IFNß-XAF1-XIAP pathway in adult mice. Toxicol Appl Pharmacol 355:247-256 (2018). PubMed: 30017639
- Jeong SI et al. XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis. Cell Death Dis 9:806 (2018). PubMed: 30042418