Application
Western blot
Sample
Rat Tissue lysate - other (Spinal cord)
Loading amount
20 µg
Specification
Spinal cord
Treatment
4 hours after 300kdyne spinal cord injury
Gel Running Conditions
Reduced Denaturing (4-20% Tris-HCl gel)
Blocking step
Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBST
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/2000
Detection
Detection method
Thermoscientific supersignal west femto
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 130, 80, 20 kDa
Positive control
Human glioma lysate
Negative control
Water
Additional data
Additional Notes
Lanes from left to right:
1. Negative control (water)
2-3. Positive control (human glioma lysate)
4-6. Rat spinal cord cytoplasmic fraction
7. Ladder- molecular weights 209, 124, 80, 49, 35, 29, 21 kda
1. Negative control (water)
2-3. Positive control (human glioma lysate)
4-6. Rat spinal cord cytoplasmic fraction
7. Ladder- molecular weights 209, 124, 80, 49, 35, 29, 21 kda
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Kelly Atkins
Verified customer
提交于 Oct 06 2010