小鼠MBL ELISA试剂盒(ab208343)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.5 pg/ml
- Range: 39.063 pg/ml - 2500 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠MBL ELISA试剂盒
参阅全部 Mannan Binding Lectin/MBL 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Mouse serum 5 7.3% 批次间 样品 n Mean SD CV% Mouse serum 4 14% -
样品类型
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
8.5 pg/ml -
范围
39.063 pg/ml - 2500 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 79.5 75% - 86.8% Cell culture media 99.7 95.4% - 103.3% Hep Plasma 96.6 88.8% - 101.2% EDTA Plasma 94.9 74.1% - 102.3% Cit plasma 102.7 90.8% - 117.3% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Mouse
不与反应: Goat, Cow, Pig -
产品概述
Mouse MBL ELISA Kit (ab208343) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MBL protein in cell culture supernatant, serum, plasma, tissue extracts, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MBL with 8.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
MBL is a secreted calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Mouse MBL Capture Antibody 1 x 600µl 10X Mouse MBL Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse MBL Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
组织特异性
Plasma protein produced mainly in the liver. -
疾病相关
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
序列相似性
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
细胞定位
Secreted. - Information by UniProt
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别名
- COLEC 1
- COLEC1
- Collectin-1
see all -
数据库链接
- Entrez Gene: 17195 Mouse
- SwissProt: P41317 Mouse
- Unigene: 30045 Mouse
相关产品
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Alternative Versions
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Related Products
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: serum 1:83, plasma (citrate) 1:83, plasma (EDTA) 1:167, plasma (heparin) 1:167. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 169.0 ng/mL in serum, 116.5 ng/mL in plasma (citrate), 179.8 ng/mL in plasma (EDTA) and 148.4 ng/mL in plasma (heparin).
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Mouse liver cells were cultured for 5 days in a medium and supernatant sample was prepared. The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 4.3 ng/mL in liver supernatant.
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The concentrations of MBL were measured in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 1.56 ng/mL in liver extract sample.
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The concentrations of MBL were measured in three different dilutions in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of MBL per mg of extract (mean +/- SD, n=3).
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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