Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
Synthetic peptide corresponding to a region within the internal amino acids 324-373 (KTTTWERPLP PGWEKRTDPR GRFYYVDHNT RTTTWQRPTA EYVRNYEQWQ) of Human Wwp2 (NP_008945).
- Transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Wwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
- AIP2 antibody
- Atrophin 1 interacting protein 2 antibody
- NEDD4 like E3 ubiquitin protein ligase antibody
Western blot - Anti-Wwp2 antibody (ab86544)
Anti-Wwp2 antibody (ab86544) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
99 kDaObserved band size:
>70 kDa (why is the actual band size different from the predicted?
Gel concentration: 6-18%
has not yet been referenced specifically in any publications.
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