Anti-Wwp2抗体(ab103527)

概述

  • 产品名称Anti-Wwp2抗体
    参阅全部 Wwp2 一抗
  • 描述
    兔多克隆抗体to Wwp2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 200 and 250 of Human Wwp2 (NP_008945.2).

  • 阳性对照
    • HeLa, 293T and mouse NIH3T3 whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab103527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性Wwp2 is a HECT domain containing E3 ubiqutin protein ligase. Wwp2 specifically interacts with Rpb1 and is responsible for targeting it for ubiquitination.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • AIP2 antibody
    • Atrophin 1 interacting protein 2 antibody
    • NEDD4 like E3 ubiquitin protein ligase antibody
    • WW domain-containing protein 2 antibody
    • Wwp 2 antibody
    see all

Anti-Wwp2 antibody 图像

  • All lanes : Anti-Wwp2 antibody (ab103527) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T at 50 µg
    Lane 5 : NIH3T3 at 50 µg

    Developed using the ECL technique

    Predicted band size : 99 kDa


    Exposure time : 3 minutes
  • Detection of Wwp2 by Western Blot of Immunprecipitate.
    Lane 1: ab103527 at 1µg/ml staining Wwp2 in HeLa whole cell lysate immunoprecipitated using ab103527 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-Wwp2 antibody (ab103527)参考文献

ab103527 has not yet been referenced specifically in any publications.

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