概述

  • 产品名称Anti-WWP1抗体
    参阅全部 WWP1 一抗
  • 描述
    兔多克隆抗体to WWP1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human WWP1.

    (Peptide available as ab43790.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: MCF7 HeLa

性能

应用

Our Abpromise guarantee covers the use of ab43791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

靶标

  • 相关性WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
  • 细胞定位Cell Membrane, Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • AIP 5 antibody
    • AIP5 antibody
    • Atropin-1-interacting protein 5 antibody
    • hSDRP1 antibody
    • Nedd 4 like ubiquitin protein ligase antibody
    • nedd4 like antibody
    • NEDD4-like E3 ubiquitin-protein ligase WWP1 antibody
    • TGIF interacting ubiquitin ligase 1 antibody
    • Tiul1 antibody
    • WW domain containing E3 ubiquitin protein ligase 1 antibody
    • WW domain-containing protein 1 antibody
    • WWP 1 antibody
    see all

Anti-WWP1 antibody 图像

  • All lanes : Anti-WWP1 antibody (ab43791) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa
    Additional bands at : 12.5 kDa,36 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab43791 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43791, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

Anti-WWP1 antibody (ab43791)参考文献

This product has been referenced in:
  • Anindya R  et al. Damage-induced ubiquitylation of human RNA polymerase II by the ubiquitin ligase Nedd4, but not Cockayne syndrome proteins or BRCA1. Mol Cell 28:386-97 (2007). Read more (PubMed: 17996703) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SAOS-2 OSTEOSARCOMA)
Specification SAOS-2 OSTEOSARCOMA
Fixative Paraformaldehyde
Permeabilization Yes - PBS TRITON 0.25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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提交于 May 03 2012

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