概述

  • 产品名称Anti-WWP1抗体
    参阅全部 WWP1 一抗
  • 描述
    兔多克隆抗体to WWP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 200 - 250 of Human WWP1 (NP_008944.1).

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab104440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
  • 细胞定位Cell Membrane, Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • AIP 5 antibody
    • AIP5 antibody
    • Atropin-1-interacting protein 5 antibody
    • hSDRP1 antibody
    • Nedd 4 like ubiquitin protein ligase antibody
    • nedd4 like antibody
    • NEDD4-like E3 ubiquitin-protein ligase WWP1 antibody
    • TGIF interacting ubiquitin ligase 1 antibody
    • Tiul1 antibody
    • WW domain containing E3 ubiquitin protein ligase 1 antibody
    • WW domain-containing protein 1 antibody
    • WWP 1 antibody
    see all

Anti-WWP1 antibody 图像

  • All lanes : Anti-WWP1 antibody (ab104440) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 105 kDa


    Exposure time : 30 seconds
  • Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.

    Detection by chemiluminescence with an exposure time of 10 seconds.

Anti-WWP1 antibody (ab104440)参考文献

ab104440 has not yet been referenced specifically in any publications.

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